تعداد اعضاء : 3503 نفر
بازدیدهای امروز :197 بار
تعداد کل بازدیدها : 703128 بار
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Volume: 41 Year: 2019 Month: 3
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Subject
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Others
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Title
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The effect of 14 weeks of endurance training with two different Intensity on serum irisin level, gene expression of skeletal muscle PGC1-α and FNDC5 and subcutaneous adipose tissue UCP1 in obese rats
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Authors
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Mahbanou Ghaderi; Hamid Mohebbi; Bahram Soltani;
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Abstract
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Background: Irisin is a new myokine that has a beneficial effects of exercise on energy metabolism. However, irisin response to different intensities of endurance training is not completely understood.
Methods: In the current experimental study, 18 male Wistar rats were fed a high-fat diet and 6 rats were nourished a standard diet for 12 weeks. After 12 weeks, obese rats were randomly divided into 3 groups: high-intensity training (34 m/min ~85% VO2max), moderate-intensity training (28 m/min ~70% VO2max) and control. Exercise groups trained 5 days/week for 12 weeks on a rodent treadmill. Serum irisin level and gene expression changes were measured using ELISA and Real Time-PCR method, respectively.
Results: In high and moderate-intensity endurance training expression of PGC1-α (P=0.001 and P=0.001), FNDC5 (P=0.013 and P=0.049) and UCP1 (P=0.001 and P=0.001) were significantly more than the control group. Serum irisin level only after high-intensity training was more than the control group (P=0.014). A positive correlation was observed between serum irisin and UCP1 in both of high (P=0.017) and moderate-intensity (P=0.046) training groups.
Conclusion: High-intensity endurance training has a positive effect on the level of serum irisin. Also, there is a positive correlation between irisin and UCP1 gene expression in subcutaneous adipose tissue. However, irisin isn’t the only factor affecting on exercise-induced UCP1 gene expression.
Keyword: Exercise training, FNDC5 protein, Peroxisome proliferator-activated receptor gamma coactivator1-alpha, Uncoupling protein 1.
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ISSN 1608-5671 (Print)
ISSN 2008-160X (Online)
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