تعداد اعضاء : 3503 نفر
بازدیدهای امروز :80 بار
تعداد کل بازدیدها : 703011 بار
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Volume: 40
Year: 2018
Month: 12
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Others
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| Title
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Polymorphisms of T-cell immunoglobulin Domain-1 Gene and its Association with Total Serum Immunoglobulin E Levels in Patients with Asthma
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| Authors
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Farzad Alizadeh Mofrad; Farid Dolatshahi; Mostafa Kolahi; Sabar Aghamohammadi;
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| Abstract
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Background: Many immunologic and inflammatory mechanisms play a role in asthma etiology. A member of a T cell immunoglobulin (Ig) domain and mucin domain (TIM) gene family, TIM-1, in 5q31-33 region is shown to be correlated with development of T helper-2 (TH2) Cells and allergic diseases. So the aim of this study was to investigate the association of the genotype and allele frequencies of the TIM-1polymorphisms with asthma and the relationships among this polymorphism to IgE levels in patients with asthma.
Methods: PCR-RFLP (Polymerase chain reaction–restriction fragment length polymorphism) and polyacrylamide gel electrophoresis (PAGE) were used for investigating the presence of TIM-1 polymorphisms in 200 asthmatic patients and 200 healthy controls. To assess the frequency of genotypes and its relationship with serum immunoglobulin E and asthma, chi-square test was used and P<0/05 was considered significant.
Results: The genotype and allele frequency of TIM-1 polymorphisms were significantly different between the patient and controls. Significant association was observed between this polymorphism and the risk of asthma.
Conclusion: The 5529A>G, 416G>C and 5529A>G polymorphismS of TIM1 gene may be associated the susceptibility of asthma and also with total serum IgE levels.
Keywords: Asthma, TIM-1, Polymorphism, Serum total immunoglobulin E.
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ISSN 1608-5671 (Print)
ISSN 2008-160X (Online)
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